Ostrom Lab


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Original Articles

Shailesh R. Agarwal, Chase Fiore, Kathryn Miyashiro, Rennolds S Ostrom, and Robert D. Harvey. Effect of adenylyl cyclase type 6 on localized production of cAMP by ß2-adrenergic receptors in human airway smooth muscle cells.  J Pharmacol Exp Thera, in press. doi: https://doi.org/10.1124/jpet.119.256594

Rinzhin T. Sherpa, Ashraf M. Mohieldin, Rajasekharreddy Pala, Dagmar Wachten, Rennolds S Ostrom, Surya M. Nauli. Sensory primary cilium is a responsive cAMP compartment distinct from the cell body.  Scientific Reports, in press. doi: 10.1038/s41598-019-43002-2

Ojiaku CA, Chung E, Parikh V, Williams JK, Schwab A, Fuentes AL, Corpuz ML, Lui V, Paek S, Bexiga NM, Narayan S, Nunez FJ, Ahn K, Ostrom RS, An SS, Panettieri RA Jr.  TGF-β1 Decreases β2-Agonist-Induced Relaxation in Human Airway Smooth Muscle. Am J Respir Cell Mol Biol. 2019 Feb 11. doi: 10.1165/rcmb.2018-0301OC

Timothy B. Johnstone, Kaitlyn H. Smith, Cynthia J. Koziol-White, Fengying Li, Austin G. Kazarian, Maia L. Corpuz, Maya Shumyatcher, Frederick J. Ehlert, Blanca E. Himes, Reynold A. Panettieri, Jr. and Rennolds S Ostrom.  PDE8 is expressed in human airway smooth muscle and selectively regulates cAMP signaling by ß2AR-AC6. Am J Respir Cell Mol Biol, 58(4):530-541, 2018.

Mavanoor R Nagaraja, Nivedita Tiwari, Shwetha K Shetty, Amarnath S Marudamuthu, Liang Fan, Rennolds S Ostrom, Jian Fu, Venkadesaperumal Gopu, Steven Idell and Sreerama Shetty. p53 expression in lung fibroblasts is linked to mitigation of fibrotic lung remodeling. Am J Pathology, 188(10):2207-2222, 2018.

Marwa M. Qadri, Ling X. Zhang, Rennolds S Ostrom, Gregory D. Jay and Khaled A. Elsaid.  cAMP Attenuates TGF-ß's Profibrotic Response in Osteoarthritic Synovial Fibroblasts: Involvement of PRG4 and Hyaluronan. Am J Physiol, Cell Physiol, 315(3):C432-C443, 2018.

Shailesh R. Agarwal, Kathryn Miyashiro, Htun Latt, Rennolds S Ostrom, and Robert D. Harvey.  Compartmentalized cAMP responses to prostaglandin EP2 receptor activation in human airway smooth muscle cells.  Br J Pharmacol, Aug;174(16):2784-2796, 2017.

Ailian Du, Shiqian Huang, Xiaonan Zhao, Kuan Feng, Shuangyan Zhang, Jiefang Huang, Xiang Miao, Fulvio Baggi, Rennolds S Ostrom, Xiangjun Chen, Yanyun Zhang and Congfeng Xu.  Suppression of CHRN endocytosis by carbonic anhydrase CAR3 in the pathogenesis of myasthenia gravis.  Autophagy,13(11):1981-1994, 2017.

Bin Wang, Yu Liu, Lianyan Huang, Ruishan Wang, Jing Jing Li,  Young-Don Kwak, Shiyong Diao, Yupin Chen, Jianjun Chen, Eunhee Kim, Carles Justicia, Dehna M. Fong, Deborah Young, Kazuko Sakata, Hao Chen, Anna Planas, Rennolds S Ostrom, Wei Li, Guang Yang, William E. Armstrong, Michael P. McDonald, Ruihong Chen, Detlef Heck & Francesca-Fang Liao. A CNS-permeable Hsp90 inhibitor rescues synaptic dysfunction and memory loss in Alzheimer's mice via an HSF1-mediated mechanism.  Molecular Psychiatry, 22(7):935, 2017.

Bogard AS, Birg AV, Ostrom RS. Non-raft adenylyl cyclase 2 defines a cAMP signaling compartment that selectively regulates IL-6 expression in airway smooth muscle cells: differential regulation of gene expression by AC isoforms. Naunyn Schmiedebergs Arch Pharmacol. 387(4):329-39, 2014       

Cai W, Du A, Feng K, Zhao X, Qian L, Ostrom RS, Xu C.  Adenylyl Cyclase 6 Activation Negatively Regulates TLR4 Signaling through Lipid Raft-Mediated Endocytosis.  J Immunol, 191(12):6093-100, 2013.         

Conley JM, Brand CS, Bogard AS, Pratt EP, Xu R, Hockerman GH, Ostrom RS, Dessauer CW, Watts VJ.  Development of a high-throughput screening paradigm for the discovery of small-molecule modulators of adenylyl cyclase: identification of an adenylyl cyclase 2 inhibitor. J Pharmacol Exp Ther. 347(2):276-87, 2013.     

Amy S. Bogard, Piyatilake Adris and Rennolds S Ostrom. Adenylyl cyclase 2 (AC2) selectively couples to EP2 receptors while adenylyl cyclase 3 (AC3) is not receptor regulated in airway smooth muscle. J Pharmacol Exp Thera, 342(2):586-95, 2012.     

Amy S. Bogard, Congfeng Xu and Rennolds S Ostrom. Human bronchial smooth muscle cells express adenylyl cyclase isoforms 2, 4 and 6 in distinct membrane microdomains. J Pharmacol Exp Thera, 337(1): 209-17, 2011.       

Xiaoqiu Liu, Fengying Li, Shu Qiang Sun, Muthusamy Thangavel, Joseph Kaminsky, Louisa Balazs and Rennolds S Ostrom. Fibroblast specific expression of AC6 enhances ß-adrenergic and prostacyclin signaling and blunts bleomycin-induced pulmonary fibrosis.  Am J Physiol Lung Cell Mol Physiol, 298(6): L819-29, 2010.          

Muthusamy Thangavel, Xiaoqiu Liu, Shu Qiang Sun, Joseph Kaminsky and Rennolds S Ostrom. The C1 and C3 domains target human type 6 adenylyl cyclase to lipid rafts and caveolae.  Cell Signalling, 21:301-308, 2008.       

Xiaoqiu Liu, Muthusamy Thangavel, Shu Qiang Sun, Joseph Kaminsky, Penden Mahautmr, Jeremiah Stitham, John Hwa and Rennolds S Ostrom. Adenylyl cyclase type 6 overexpression selectively enhances beta-adrenergic and prostacyclin receptor-mediated inhibition of cardiac fibroblast function because of colocalization in lipid rafts. Naunyn Schmiedebergs Arch Pharmacol, 377(4-6):359-69, 2007.       

Xiaoqiu Liu, Shu Qiang Sun, Aviv Hassid and Rennolds S Ostrom.  cAMP inhibits TGF-ß-stimulated collagen synthesis via inhibition of ERK1/2 and Smad signaling in rat cardiac fibroblasts.  Mol Pharmacol, 70:1992-2003, 2006.          

Liu X, Sun SQ, Ostrom RS. Fibrotic pulmonary fibroblasts show blunted inhibition by cAMP due to deficient CREB phosphorylation. J Pharmacol Exp Thera, 315(2):678-87, 2005.       

Ostrom RS, Bundey RA, Insel PA. Nitric oxide inhibition of adenylyl cyclase type 6 activity is dependent upon lipid rafts and caveolin signaling complexes. J Biol Chem, 279(19): 19846-53, 2004.     

Liu X, Ostrom RS, Insel PA. Cyclic AMP-elevating agents and adenylyl cyclase over-expression promote an anti-fibrotic phenotype in pulmonary fibroblasts. Am J Physiol Cell Physiol, 286(5):C1089-99, 2004.     

Ostrom RS, Naugle JE, Hase M, Gregorian C, Swaney JS, Insel PA, Brunton LL, Meszaros JG. Angiotensin II enhances adenylyl cyclase signaling via Ca2+/calmodulin. Gq-Gs cross-talk regulates collagen production in cardiac fibroblasts. J Biol Chem, 278(27): 24461-8, 2003.       

Loomis WH, Namiki S, Ostrom RS, Insel PA, Junger WG. Hypertonic Stress Increases T Cell Interleukin-2 Expression through a Mechanism That Involves ATP Release, P2 Receptor, and p38 MAPK Activation, J Biol Chem, 278(7):4590-6, 2003.     

Ostrom RS, Liu X, Gregorian C, Drenan RM, Seasholtz TM, Insel PA. Compartmentation of adenylyl cyclase isoform expression and receptor coupling in aortic smooth muscle cells, Mol Pharmacol , 62:983-992, 2002.     

Zheng B, Ma Y-C, Ostrom RS, Lavoie C, Gill GN, Insel PA, Huang X-Y, Farquhar MG. RGS-PX1: A gap for G(s and a sorting nexin in vesicular trafficking, Science, 294:1939-1942, 2001.       

Ostrom RS, Gregorian C, Drenan RM, Xiang Y, Regan JW, Insel PA. Receptor number and caveolar co-localization determine receptor coupling efficiency to adenylyl cyclase, J Biol Chem , 276(45):42063-9, 2001     

Ostrom RS, Gregorian C, Drenan RM, Gabot K, Rana BK, Insel PA. Key role for constitutive cyclooxygenase-2 of MDCK cells in basal signaling and response to released ATP, Am J Physiol: Cell Physiol, 281:C524-C531, 2001.     

Ostrom RS, Gregorian C, Insel PA. Cellular release of and response to ATP as key determinants of the set-point of signal transduction pathways J Biol Chem, 275:11735-11739, 2000.     

Ostrom RS, Violin JD, Coleman S, Insel PA. Selective enhancement of ß-adrenergic receptor signaling by overexpression of adenylyl cyclase type 6: co-localization of receptor and adenylyl cyclase in caveolae of cardiac myocytes. Mol Pharmacol, 57:1075-1079, 2000.     

Book Chapters, Invited Publications and Review Articles

Reynold A. Panettieri, Yassine Amrani, Cynthia Koziol-White, Rennolds S Ostrom and Omar Tliba. Non-Genomic Effects of Glucocorticoids: An Updated View. Trends Pharmacol Sci, 40(1):38-49, 2019.

Timothy Johnstone, Shailesh Agarwal, Robert Harvey and Rennolds Ostrom.  cAMP Signaling Compartmentation: Adenylyl Cyclases as Anchors of Dynamic Signaling Complexes.  Mol Pharmacol, 93(4):270-276, 2018.

Carmen Dessauer, Val Watts, Rennolds Ostrom, Marci Conti, Stephan Dove and Roland Seifert. International Union of Basic and Clinical Pharmacology. CI. Structures and Small Molecule Modulators of Mammalian Adenylyl Cyclases.  Pharmacol Rev 69(2):93-139, 2017.    

Shailesh R. Agarwal, Rennolds S Ostrom and Robert D. Harvey.  Membrane microdomains and cAMP compartmentation in Cardiac Myocytes.  Cardiac and Vascular Biology: Microdomains of the Cardiovascular System, Viacheslav Nikolaev and Manuela Zaccolo (editors), Springer Nature, 2017.

Rennolds S Ostrom. A two-pronged weapon in the fight against fibrosis. Focus on "Inhibition of Wnt/β-catenin signaling promotes epithelial differentiation of mesenchymal stem cells and repairs bleomycin-induced lung injury". Am J Physiol Cell Physiol 307(3):C232-3, 2014.

Rennolds S Ostrom.  A new molecular target for blunting organ fibrosis. Focus on "Secreted Frizzled-related protein 2 as a target in antifibrotic therapeutic intervention". Am J Physiol Cell Physiol. 306(6):C527-82014.     

Rennolds S Ostrom, Amy S. Bogard, Robert Gros and Ross D. Feldman.  Choreographing the adenylyl cyclase signalosome: sorting out the partners and the steps. Naunyn-Schmiedebergs Arch Pharmacol, 385(1):5-12, 2012.     

Radomir M. Slominski, Russel J. Reiter, Natalia Schlabritz-Loutsevitch, Rennolds S Ostrom and Andrzej T. Slominski. Melatonin Membrane Receptors in Peripheral Tissues: Distribution and Functions. Mol Cell Endocrin, 351:152-166, 2012.    

Hemal H. Patel and Rennolds S Ostrom.  An orphan GPCR finds a home in the heart.  Am J Physiol Cell Physiol, 295(2):H479-81, 2008          

Rennolds S Ostrom and Xiaoqiu Liu. Detergent and detergent-free methods to define lipid rafts and caveolae.  Methods in Molecular Biology, vol. 400: Methods in Membrane Lipids.  A.M. Dopico, ed.  Humana Press Inc., Totowa, NJ, 2008.          

Rennolds S. Ostrom and Paul A. Insel. Methods for the study of signaling molecules in membrane lipid rafts and caveolae. Methods in Molecular Biology, vol. 332: Transmembrane Signaling Protocols, 2nd Edition.  Ali and Haribabu, ed.  Humana Press Inc., Totowa, NJ, 2006.         

Insel PA, Head BP, Ostrom RS, Patel HH, Swaney JS, Tang CM and Roth DM.  Caveolae and lipid rafts: G protein-coupled receptor signaling microdomains in cardiac myocytes.  Ann N Y Acad Sci, 1047:166-172, 2005.     

Ostrom RS. Caveolins muscle their way into the regulation of cell differentiation, development, and function. Focus on "Muscle-specific interaction of caveolin isoforms: differential complex formation between caveolins in fibroblastic vs. muscle cells."  Am J Physiol: Cell Physiol, 288:C507-C509, 2005.       

Ostrom RS, Insel PA.  The evolving role of lipid rafts and caveolae in G protein-coupled receptor signaling: Implications for molecular pharmacology.  Brit J Pharmacol, 143(2):235-45, 2004.       

Ostrom RS and Insel PA. Forskolin as a tool for examining adenylyl cyclase expression, regulation and G protein signaling. Cell Mol Neurobiol, 23(3):305-1, 2003.    

Ostrom RS, Rana BK and Insel PA. Stoichiometry of G Protein-coupled Receptor Signaling: Implications in the Genomic Era. Pharmaceutical News, 9:295-303, 2003.

Ostrom RS. New determinants of receptor-effector coupling: trafficking and compartmentation in membrane microdomains. Mol Pharmacol, 61(3):473-6, 2002.    

Insel PA, Ostrom RS, Zambon AC, Hughes RJ, Balboa MA, Shehnaz D, Gregorian C, Torres B, Firestein BL, Xing M, Post SR. P2Y receptors of MDCK cells: epithelial cell regulation by extracellular nucleotides. Clin Exp Pharmacol Physiol, 28(4):351-4 , 2001.    

Ostrom RS, Post SR and Insel PA. Stoichiometry and compartmentation in G protein-coupled receptor signaling: implications for therapeutic interventions involving Gs. J Pharmacol Exp Ther , 294(2):407-412, 2000.    

Post SR, Ostrom RS and Insel PA. Biochemical methods for detection and measurement of cyclic AMP and adenylyl cyclase activity . Methods Mol Bio 126:363-74, 2000.

Ostrom RS and Insel PA. Caveolar microdomains of the sarcolemma: compartmentation of signaling molecules comes of age. Circ Res , 84(9):1110-2, 1999.